DMMC Course: TECHNIQUES & STRATEGIES IN MOLECULAR MEDICINE
1015-1100 Monday 10 December 2007, Panoz institute, LTEE2, TCD
Differential
gene expression: overview of relevant methods
Prof
William Gallagher (UCD Conway Institute of
Biomolecular & Biomedical Research)
This lecture will summarise the main approaches used to determine alterations in gene expression at the RNA level. Emphasis will be placed in this context on global approaches that attempt to map differences in the transcriptome, i.e. entire complement of transcripts in a cell. Methodologies that will be addressed include differential display, subtractive hybridization, high-throughput sequencing (ESTs and SAGE), and DNA microarray technologies. Key examples from the literature will be utilised to illustrate examples of investigators applying these technologies to understand biological phenomena, with a focus on disease-related processes. An indication of the relevant infrastructure and expertise to carry out this work within the DMMC will be presented.
Review articles
Lennon, G. G.
(2000).
High-throughput gene expression analysis for drug discovery.
Drug Discovery Today, 5, 59-66.
Schulze, A. and
Downward, J. (2001).
Navigating gene expression using microarrays – a technology
review.
Nature Cell Biology, 3, E190-E195.