DMMC
Course: TECHNIQUES & STRATEGIES IN MOLECULAR MEDICINE
0930-1015
Tuesday 11 December 2007, Panoz institute, LTEE2, TCD
Introduction to cloning
Dr Ross McManus (Institute of
Molecular Medicine, TCD)
Even in the post genome era, DNA cloning is essential to the manipulation
and stable propagation of genetic material. This talk will cover the basic
aspects of DNA cloning, ranging from the anatomy of cloning vectors to the
choice of vectors based on the cloning strategy employed. The strategy employed
will depend on the overall objectives of the project and the nature of the
starting information or material available. Thus different choices and approaches
would be employed for a sequencing project compared with a genome mapping
project or production of RNA or protein. I will discuss a number of basic
and specialised cloning strategies to illustrate some of the options and possibilities
available.