DMMC Course: TECHNIQUES & STRATEGIES IN MOLECULAR MEDICINE

1130-1215 Tuesday 11 December 2007, Panoz institute, LTEE2, TCD

Transgenics and knockouts
Dr Derek Brazil (UCD Conway Institute of Biomolecular & Biomedical Research)

This lecture will provide a broad overview of the strategies used to generate both transgenic and knockout mice, starting from the generation of the DNA constructs using cDNAs or genomic DNA, and proceeding through embryonic stem cell biology, to aggregation and chimeric mouse generation. Details on genotyping of transgenic animals, as well as phenotype characterization will be discussed. Specific examples such as the IRS-2 knockout will be cited.