DMMC Course: TECHNIQUES & STRATEGIES IN MOLECULAR MEDICINE

1015-1100 Thursday 13 December 2007, Panoz institute, LTEE2, TCD

Cell imaging and sorting – flow cytometry

Dr William Watson (UCD Conway Institute of Biomolecular & Biomedical Research)

Flow cytometry is a method for quantitating components or structural features of cells, primarily by optical means. Although it makes measurements on one cell at a time, it can process thousands of cells in a few seconds. Since cell types can be distinguished by quantitating structural features, flow cytometry can be used to count prokaryotic or eukaryotic cells of different types in complex mixtures.

Flow cytometry overview